Subjects: Biology >> Ecology submitted time 2017-11-29 Cooperative journals: 《中国生态农业学报》
Abstract： Anthocyanins are important antioxidant molecules that help protect plants from reactive oxygen species (ROS) damage. Especially under adversity conditions, the regulation of Suc in plants depends on its ability to induce the accumulation of anthocyanins. To reveal the intrinsic relationship between photosynthetic and anthocyanin-regulated pathways of the over-expressing maize C4-phosphoenolpyruvate carboxylate (PEPC, EC 4.1.1.31) gene rice (PC) in drought tolerance, PC and untransformed wild-type (WT) acted as materials, were treated with 50 μmol∙L-1 photosynthetic inhibitor DCMU for 1h, and the performance of the rice seedlings in the 4-5 leaf stage was observed under 12% PEG-6000 simulated drought treatment. The results showed that the relative water content of WT and PC was significantly reduced under DCMU treatment, and PC was significantly higher than WT under 12% PEG-6000 simulated drought treatment, which was accompanied by the higher anthocyanin content in PC lines. From the photosynthetic data, compared with 12% PEG-6000 alone, DCMU combined with 12% PEG-6000 significantly inhibited the net photosynthetic rate, stomatal conductance, intercellular CO2 and carboxylation efficiency of the two rice lines, but the PC lines was significantly higher than WT. Meanwhile, DCMU combined with 12% PEG-6000 treatment down-regulated the content of endogenous sucrose in the two materials, but the sucrose content in PC lines was significantly higher than that in WT. Further studies showed that higher sucrose levels in PC were associated with higher levels of transcription with, bHLH(OsB1, OsB2), R2R3-MYB(OsC1), COP1(Constitutively photomorphogenic 1), HY5(Elongated hypocotyl 5), OsPAL, OsCHI, OsCHS, OsF3H, OsF3’H, OsDFR, OsANS to synthesize more anthocyanin, it can synthesize more anthocyanins to enhance water retention capacity. In addition, PC rice can sense drought signals through NO and Ca2+, which involved in the regulation of transcription factors, and then participate in the regulation of anthocyanin synthesis gene, synthesis of more anthocyanin, thereby enhancing the response of PC rice under drought stress to enhance the water retention capacity, keep the photosynthetic capacity stable and finally bear drought. Therefore, it is beneficial in the molecular breeding of “C4 Rice” and the study the symphony between high yield and resistance in plants to open a new vision.
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